caspARREST Caspase-3 Inhibitor Drug Screening Kit
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| Catalog Number | GTX85560 |
| Product Name | caspARREST Caspase-3 Inhibitor Drug Screening Kit |
| Full Name | Screening kit for caspase-3 inhibitors |
| Synonyms | Caspase-3 Assay, Caspase 3 Activity Assay, Caspase substrate kit, Apoptosis Assay, Caspase-3 Substrate, Caspase-3 Activity Assay, Caspase3 Substrate, Caspase 3 Substrate, Caspase3 Assay, Caspase3 Activity Assay, Caspase 3 Assay |
| Product Description | Screening kit for caspase-3 inhibitors |
| Background | Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts. The Caspase-3 Inhibitor Drug Screening Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate DEVD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase-3 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase-3 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors. ‧ Detection method- Fluorecence microtiter plate reader (400-nm excitation filter and 505-nm emission filter) and fluorometer ‧ Sample type- Cell samples treated with Caspase inhibitor drugs ‧ Species reactivity- Mammalian ‧ Kit size- 100 assays ‧ Applications- Effective means for screening caspase inhibitors using fluorometric methods.Features and Benefits ‧ Simple one-step procedure; takes only 1-1.5 hours ‧ Fast and convenient ‧ Inhibition of caspase-3 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.Kit Contents: 2X Reaction Buffer Caspase Substrate DEVD-AFC (1 mM) DTT (1 M) Active Caspase-3 (Lyophilized) Caspase-3 Inhibitor |
| Species Reactivity | Mammal |
| Storage Instruction | Store at -20°C. Product has an expected shelf life of 6-12 months. |
| Notes | For research use only. Not intended for any diagnostic or therapeutic purpose. |
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Drug Screening kits: caspARREST Caspase-3 Inhibitor