Buffer untuk Heat Induced Epitope Retrieval ?

Metode Antigen Retrieval dengan pemanasan dan tekanan (HIER) sangat dipengaruhi pula keefektifannya dengan buffer yang digunakan. Beberapa penelitian menunjukkan bufer-bufer dengan pH basa lebih efektif untuk antibodi tertentu, sehingga sangat diperlukan pemilihan yang tepat antara antibody dan jenis buffer yang diperlukan.

Dibawah ini adalah buffer yang telah dioptimasi dengan beberapa kondisi dan kebutuhan. semua reagensia dibawah adalah produk Biocare Medical dari USA, dan anda dapat memperolehnya di www.biozatix.com

Product Name Intended Purpose Composition pH Usage Instructions
Diva Decloaker Heat retrieval only (Proprietary) 6.2 Concentrate is diluted in DI water; non-flammable and non-toxic; no deparaffinization
Rodent Decloaker Heat retrieval only (Proprietary) 6.0 Diluted in DI water; non-flammable and non-toxic; no deparaffinization
Reveal Heat retrieval only Citrate-based 6.0 Concentrate is diluted in DI water; non-flammable and non-toxic; capable of deparaffinizing, but if used for this purpose, must be used in conjunction with Hot Rinse
Borg Decloaker Heat retrieval only Tris-based 9.5 Used after deparaffinization; non-flammable and non-toxic; capable of deparaffinizing, but if used for this purpose, must be used in conjunction with Hot Rinse
Universal Decloaker Heat retrieval only (Proprietary) 6.0 Diluted in DI water; non-flammable and non-toxic; no deparaffinization
Antigen Decloaker Heat retrieval only Citrate-based 6.0 Concentrate is diluted in DI water; non-flammable and non-toxic; no deparaffinization
Bull’s Eye Decloaker Heat retrieval only (Proprietary) 6.0
EDTA Decloaker Heat retrieval only EDTA-based 8.5 Diluted in DI water; non-flammable and non-toxic; no deparaffinization
Nuclear Decloaker Heat retrieval only Tris-based 9.5 Concentrate is diluted in DI water; non-flammable and non-toxic; no deparaffinization
Buffer untuk Heat Induced Epitope Retrieval ?
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